Capillary Electrophoresis: Instrumentation and Operation by Wim Kok

By Wim Kok

In this quantity the overall rules of capillary (zone) electrophoresis, the functionality of this separation solution to be anticipated, the instrumentation and methods to be had and sensible instructions for the operaton of CE tools should be mentioned. The emphasis might be at the regimen program of CE with commercially to be had tools, yet cognizance may also be paid to devolpments nonetheless in a examine level and their attainable implications for the future.

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Nielen, 1. High Resolut. , 16 (1993) 62. S. Lee, We. -T. Wu, Anal. Chem. 63 (1990) 1550. A. G. Ewing, Anal. Chem. 64 (1992) 512. A. R. M. van de Goor and D. McManigill,1. A, 652 (1993) 283. S. -T. Wu, T. Lopes and B. Patel, 1. , 559 (1991) 133. S. Bello, L. G. Righetti, 1. 1 The role ofthe background electrolyte in CZE In capillary zone electrophoresis (CZE), which is the most general and widely applied mode of CE, analyte ions are separated as zones migrating with different velocities through a so-called background electrolyte (BGE), a solution of a buffering salt.

24) one finds a simple formula to express the quality of a BGE type for strong analyte ions: f1A QEMD --------------- If1B - f1AI . 21 ) when A is the analyte ion, B the co-ion and C the counter-ion in the BGE. 8B the influence of the counterion is shown. 8 it appears that such buffering salts do not specifically exhibit a better quality in respect to electromigration dispersion. For analyte ions with a high mobility a fast BGE co-ion should be chosen. 8 The quality parameter of the 8GE as a function of the analyte mobility.

E. A. Lucy, Anal. , 69 (1997) 3435. [22] M. M. , 602 (1992) 227. [23] A. Dougherty, C. Wooley, D. Williams, D. Swaile, R. Cole and M. Sepaniak, 1. Liq. , 14 (1991) 907. M. Bruin, R. C. Kraak and H. Poppe, 1. , 480 (1989) 339. [25] S. Hjerten and K. Kubo, Electrophoresis, 14 (1993) 390. [26] H. A. Cunat-Walter, 1. , 716 (1995) 27. [27] M. H. K1eemiss, G. Schomburg, Anal. , 66 (1994)2038. [28] H. Bayer and H. Engelhardt, 1. , 8 (1996) 479. WF. Nielen, 1. High Resolut. , 16 (1993) 62. S. Lee, We.

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