Drosophila Cytogenetics Protocols by Daryl S. Henderson

By Daryl S. Henderson

Probably no unmarried experimental organism has contributed as a lot to our realizing of the constitution, functionality, and mechanism of transmission of eukaryotic chromosomes as has the fruit fly, Drosophila melanogaster. In Drosophila Cytogenetics Protocols, best drosophilists describe, in step by step element, the entire crucial strategies for learning Drosophila chromosomes and recommend new avenues for medical exploration. normal parts coated contain analyses of nuclei and chromosomes in spermatogenesis and oogenesis, fluorescence-activated mobilephone sorting of ovarian follicle cells, analyses of nuclear department cycles in embryos, and analyses of programmed mobile demise. furthermore, there are traditional light-, electron-, and immunofluorescence-microscopic analyses of polytene chromosomes; cytological analyses of larval brains, imaginal discs, and histoblasts; and nonfluorescent and fluorescent in situ hybridization (FISH) to either polytene and mitotic chromosomes. A state of the art mixed FISH and immunolocalization protocol may be very important for locating how DNA series impacts higher-order chromosome structure and eventually gene expression. every one comfortably reproducible protocol bargains a history creation, apparatus and reagent lists, and tips about troubleshooting and keeping off pitfalls.
quite simply available and hugely useful, Drosophila Cytogenetics Protocols offers a entire cytogenetics laboratory handbook for Drosophila investigators, person who is acceptable not just for newbies, but in addition hugely informative for professional investigators.

Show description

Read Online or Download Drosophila Cytogenetics Protocols PDF

Similar entomology books

TRUE BUGS OF THE WORLD (Hemiptera: Heteroptera)

Insects is a time period we use freely for something from a spider to a moth to a beetle to an ant. such a lot bugs should not insects. actual insects of the area describes the bugs often called the Hemiptera: Heteroptera, the biggest staff of bugs on the earth and the main varied. There are seventy five households and a few 38,000 species chanced on on all continents other than Antarctica.

The Welfare of Cattle

Animal welfare is attracting expanding curiosity around the globe, yet really from these in built international locations, who now have the information and assets on the way to provide the simplest administration structures for his or her livestock, in addition to in all likelihood having the ability to supply ample assets for spouse, zoo and laboratory animals.

Methods in cell biology. / Volume 44, Drosophila melanogaster: practical uses in cell and molecular biology

Drosophila melanogaster: sensible makes use of in mobile and Molecular Biology is a compendium of usually brief technical chapters designed to supply state-of-the artwork the right way to the wide neighborhood of telephone biologists, and to place molecular and phone organic reports of flies into standpoint. The publication makes the baroque facets of genetic nomenclature and method obtainable to cellphone biologists.

Transgenic Insects: Techniques and Applications

Insect transgenesis provides advancements in agriculture, prescribed drugs and public wellbeing and fitness. Many vital bugs can now be repeatedly remodeled with effectors that experience important functions. Agriculture offers the most important marketplace for transgenic bugs and has a foundational historical past of good fortune with sterile insect strategy for regulate of pests together with Mediterranean fruit flies and screwworms.

Extra info for Drosophila Cytogenetics Protocols

Example text

And Ostrowski, S. E. (1997) The structure of heterochromatic DNA is altered in polyploid cells of Drosophila melanogaster. Mol. Cell. Biol. 17, 1254–1263. 190. Lifschytz, E. (1983) Sequence replication and banding organization in the polytene chromosomes of Drosophila melanogaster. J. Mol. Biol. 164, 17–34. 191. Moshkin, Y. , Alekseyenko, A. , Semeshin, V. , et al. (2001) The Bithorax Complex of Drosophila melanogaster: underreplication and morphology in polytene chromosomes. Proc. Natl. Acad.

2002) Evidence for large domains of similarly expressed genes in the Drosophila genome. J. Biol. 8. 206. DuPraw, E. J. and Rae, M, P. M. (1966) Polytene chromosome structure in relation to the “folded fibre” concept. Nature 212, 598–600. 207. Sorsa, V. (1974) Organization of replicative units in salivary gland chromosome bands. Hereditas 78, 298–302. 208. D. (1980) Structural paradox of polytene chromosomes. Cell 22, 869–874. 209. Spierer, A. and Spierer, P. (1984) Similar levels of polyteny in bands and interbands of Drosophila giant chromosomes.

And Wasserman, S. (1998) Regulation and execution of meiosis in Drosophila males. Curr. Topics Dev. Biol. 37, 301–332. 3. McKim, K. , Jang, J. , and Manheim, E. A. (2002) Meiotic recombination and chromosome segregation in Drosophila females. Annu. Rev. Genet. 36, 205–232. 4. Darlington, C. D. (1937) Recent Advances in Cytology. Blackiston, Philadelphia. 5. Sutton, W. S. (1903) The chromosomes in heredity. Biol. Bull. 4, 231–251. 6. Peters, J. A. (1959) Classic Papers in Genetics. Prentice-Hall, Englewood Cliffs, NJ.

Download PDF sample

Rated 4.01 of 5 – based on 7 votes