By Guanghui Ma, Zhi-Guo Su
Microspheres and microcapsules have very extensive purposes in a variety of fields, in particular in these of biotechnology and biopharmaceuticals, as focusing on drug-delivery companies, separation media for protein, peptide, DNA, etc. it's a huge problem to layout and get ready microspheres and microcapsules of alternative sizes and constructions from a variety of fabrics and advance new innovations. This publication specializes in new Read more...
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Microspheres and Microcapsules in Biotechnology : Design, Preparation and Applications
Microspheres and microcapsules have very extensive functions in a variety of fields, specifically in these of biotechnology and biopharmaceuticals, as concentrating on drug-delivery providers, separation media for protein, peptide, DNA, and so on. it's a significant problem to layout and get ready microspheres and microcapsules of alternative sizes and buildings from quite a few fabrics and boost new recommendations.
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Additional info for Microspheres and Microcapsules in Biotechnology : Design, Preparation and Applications
Sample text
However, despite all these 13 14 Microspheres for Enzyme Immobilization advantages, this new technology is still suffering from the drawback of being tiny in scale as was mentioned with nanoparticle carrier in an earlier section of this chapter. Their tiny scale always leads to arduous task in recycling and difficulties in process control. Thus, a more sophisticated approach is required to overcome these drawbacks and to pave the way for industrialization as well. 5 Enzyme Supported by Microcapsules Covalent binding or cross-linking processes often expose enzyme molecules to harsh conditions, which might affect their native structures and activity.
6 µm diameters and trypsin was immobilized using adsorption method. 0 in 1 h. 5% of the released trypsin could be reloaded in 10 min. This study offers a promising alternative for enzyme recovery in biotechnology. Photo regulation of enzyme activities is of general interest for future bioelectronic devices. “On-off” photo-stimulation of enzyme activities was achieved by covalent attachment of photoisomerizable (photochromic) components to proteins and modification of the enzyme-active site by photoisomerization by the use of photoisomerizable inhibitors.
Therefore, it is hardly reported in literature that micronsized (several microns) magnetic polymeric supports with high density of surface functional groups could be prepared. To overcome this disadvantage, one choice is that highly porous materials with large pore size have to be employed. These materials still have the shortcomings of diffusion restriction. Yong et al. (2008) developed a new kind of magnetic porous carrier with active epoxy groups by the copolymerization of glycidyl methacrylate (GMA), ethylene glycol dimethacrylate (EGDMA), and vinyl acetate (VAc) encapsulating oleic acid–coated nanomagnetite (Fe3O4) (Fig.